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The Relation Between Promoter Chromatin Status, Xyr1 and Cellulase Expression in Trichoderma reesei

[ Vol. 17 , Issue. 2 ]

Author(s):

Thiago M Mello-de-Sousa, Alice Rassinger, Christian Derntl, Marcio J. Poças-Fonseca, Robert L Mach and Astrid R Mach-Aigner   Pages 145 - 152 ( 8 )

Abstract:


The ascomycete Trichoderma reesei is used for the production of plant cell wall-degrading enzymes in industrial scale. The interplay of the transactivator Xyr1 and the repressor Cre1 mainly regulates the expression of these enzymes. During inducing conditions, such as in the presence of sophorose, the transcription of the two major cellulase-encoding genes, cbh1 and cbh2, is activated as well as the expression of xyr1. In the presence of D-glucose carbon catabolite repression mediated by Cre1 takes place and the expression of Xyr1 and the plant cell wall-degrading enzymes is downregulated. In this study we compare the chromatin status of xyr1, cbh1, and cbh2 promoters in the wild-type strain and the Cre1-deficient strain Rut-C30. Chromatin rearrangement occurs in the xyr1 promoter during induction on sophorose. Chromatin opening and protein-DNA interactions in the xyr1 promoter were detected especially in a region located 0.9 kb upstream the translation start codon, which bears several putative Cre1-binding sites and a CCAAT-box. Moreover, the xyr1 promoter is overall more accessible in a cre1-truncated background, no matter which carbon source is present. This makes the xyr1 regulatory sequence a good target for promoter engineering aiming at the enhancement of cellulase production.

Keywords:

Cellulases, Chromatin, Promoter, Trichoderma reesei, Rut-C30, Xyr1.

Affiliation:

Department for Biotechnology and Microbiology, Institute of Chemical Engineering, TU Wien, Gumpendorfer Str. 1a, A-1060 Wien, Austria.

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