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Transcriptome Analysis of ABA/JA-Dual Responsive Genes in Rice Shoot and Root

[ Vol. 19 , Issue. 1 ]

Author(s):

Jin-Ae Kim, Nikita Bhatnagar, Soon Jae Kwon, Myung Ki Min, Seok-Jun Moon, In Sun Yoon, Taek-Ryoun Kwon, Sun Tae Kim and Beom-Gi Kim*   Pages 4 - 11 ( 8 )

Abstract:


The phytohormone abscisic acid (ABA) enables plants to adapt to adverse environmental conditions through the modulation of metabolic pathways and of growth and developmental programs. We used comparative microarray analysis to identify genes exhibiting ABA-dependent expression and other hormone-dependent expression among them in Oryza sativa shoot and root. We identified 854 genes as significantly up- or down-regulated in root or shoot under ABA treatment condition. Most of these genes had similar expression profiles in root and shoot under ABA treatment condition, whereas 86 genes displayed opposite expression responses in root and shoot. To examine the crosstalk between ABA and other hormones, we compared the expression profiles of the ABAdependently regulated genes under several different hormone treatment conditions. Interestingly, around half of the ABA-dependently expressed genes were also regulated by jasmonic acid based on microarray data analysis. We searched the promoter regions of these genes for cis-elements that could be responsible for their responsiveness to both hormones, and found that ABRE and MYC2 elements, among others, were common to the promoters of genes that were regulated by both ABA and JA. These results show that ABA and JA might have common gene expression regulation system and might explain why the JA could function for both abiotic and biotic stress tolerance.

Keywords:

Plant stress, ABA signaling, ABA responsive genes, JA responsive genes, Hormone crosstalk, Microarray analysis.

Affiliation:

Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, Jeonju 54874, Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 627-706, Gene Engineering Division, Department of Agricultural Biotechnology, National Institute of Agricultural Sciences, RDA, Jeonju 54874

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