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Implication of Gastric Cancer Molecular Genetic Markers in Surgical Practice

[ Vol. 18 , Issue. 5 ]

Author(s):

Marina V. Nemtsova, Vladimir V. Strelnikov, Alexander S. Tanas, Igor I. Bykov, Dmitry V. Zaletaev, Viktoria V. Rudenko, Alexander I. Glukhov, Tatiana V. Kchorobrich, Yi Li, Vadim V. Tarasov, George E. Barreto and Gjumrakch Aliev*   Pages 408 - 415 ( 8 )

Abstract:


Introduction: We have investigated aberrant methylation of genes CDH1, RASSF1A, MLH1, N33, DAPK, expression of genes hTERT, MMP7, MMP9, BIRC5 (survivin), PTGS2, and activity of telomerase of 106 gastric tumor samples obtained intra-operatively and 53 gastric tumor samples from the same group of patients obtained endoscopically before surgery. Biopsy specimens obtained from 50 patients with chronic calculous cholecystitis were used as a control group. Together with tissue samples obtained from different sites remote to tumors, a total of 727 samples have been studied. The selected parameters comprise a system of molecular markers that can be used in both diagnostics of gastric cancer and in dynamic monitoring of patients after surgery. Special attention was paid to the use of molecular markers for the diagnostics of malignant process in the material obtained endoscopically since the efficacy of morphological diagnostics in biopsies is compromised by intratumoral heterogeneity, which may prevent reliable identification of tumor cells in the sampling. Our data indicated that certain molecular genetic events provided more sensitive yet specific markers of the tumor.

Conclusion: We demonstrated that molecular profiles detected in preoperative biopsies were confirmed by the material obtained intra-operatively. The use of endoscopic material facilitates gastric tumors pre-operative diagnostics, improving early detection of gastric cancer and potential effective treatment strategies.

Keywords:

Gastric cancer, Genetic markers, Molecular profiles, Biopsies, Treatment, Heterogeneous., Gastric cancer, Genetic markers, Molecular profiles, Biopsies, Treatment, Heterogeneous.

Affiliation:

Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Russian Ministry of Health, Trubetskaya St. 8/2, Moscow, 119991,Department of Medical Genetics, Russian Medical Academy of Postgraduate Education, Ministry of Health of the Russian Federation, Moscow, 119991, Research Centre for Medical Genetics, Moskvorechie St. 1, 115472, Moscow, Research Centre for Medical Genetics, Moskvorechie St. 1, 115472, Moscow, Department No 1, Medical Faculty, Faculty Surgery, Sechenov First Moscow State Medical University, Russian Ministry of Health, Trubetskaya St. 8/2, Moscow, 119991, Institute of Molecular Medicine, Sechenov First Moscow State Medical University, Russian Ministry of Health, Trubetskaya St. 8/2, Moscow, 119991,Research Centre for Medical Genetics, Moskvorechie St. 1, 115472, Moscow, Research Centre for Medical Genetics, Moskvorechie St. 1, 115472, Moscow, Department of Biochemistry, Sechenov First Moscow State Medical University, Russian Ministry of Health, Trubetskaya, 8/2, Moscow, 119991,National Research Centre “Kurchatov Institute”, Moscow, 123182, Department No 1, Medical Faculty, Faculty Surgery, Sechenov First Moscow State Medical University, Russian Ministry of Health, Trubetskaya St. 8/2, Moscow, 119991, Department of Human Sciences, Texas A&M University-Kingsville, TX 78363, Institute of Pharmacy and Translational Medicine, Sechenov First Moscow State Medical University, 119991 Moscow, Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C., “GALLY” International Biomedical Research Consulting LLC, San Antonio, TX 78229,USA;School of Health Sciences, University of Atlanta, Johns Creek, GA 30097, USA; Institute of Physiologically Active Compounds, Russian Academy of Sciences, Chernogolovka, 142432

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