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Identification of tRNA-Derived Fragments Expression Profile in Breast Cancer Tissues

Author(s):

Xiaoming Wang, Yining Yang, Xuyan Tan, Xuelian Mao, Da Wei, Yufeng Yao, Pan Jiang, Dongping Mo, Ting Wang and Feng Yan*   Pages 1 - 15 ( 15 )

Abstract:


Background: In recent years, tRFs(transfer RNA-Derived Fragments) and transfer RNA-Derived Stress-induced RNAs (or tRNA halves) have been shown to have vital roles in cancer biology. We aimed to reveal the expression profile of tRNA-derived fragments in breast cancer tissues in the study, and to explore their potential as biomarkers of breast cancer.

Methods: We characterized the tRNA-derived fragments expression profile from 6 paired clinical breast cancer tissues and adjacent normal samples. Then we selected 6 significantly expressed tRNA-derived fragments and screened the genes for validation by using Quantitative Real-time PCR. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes biological pathway were finally analyzed.

Results: We found 30 differentially expressed tRNA-derived fragments across our dataset, out of which 17 were up-regulated, 13 were down-regulated. Compared with 16 clinical breast cancer tissues and adjacent normal tissues by qPCR, the results demonstrated that tRF-32-Q99P9P9NH57SJ (FC = -2.6476, p = 0.0189), tRF-17-79MP9PP (FC = -4.8984, p = 0.0276) and tRF-32-XSXMSL73VL4YK (FC = 6.5781, p = 0.0226) were significantly expressed in breast cancer tissues (p < 0.001). tRF-32-XSXMSL73VL4YK was significantly up-regulated, tRF-32-Q99P9P9NH57SJ and tRF-17-79MP9PP were significantly down-regulated which the expression patterns were similar to the sequencing results. The top ten significantly results of GO and KEGG pathways enrichment analysis were presented.

Conclusions: Our studies have demonstrated that there were significantly expressed tRNA-derived fragments in breast cancer tissues. They are hopefully to become biomarkers and would be valuable researches in this area.

Keywords:

breast cancer; tRNA-derived fragments; expression profile; biomarker

Affiliation:

Jiangsu Institute of Cancer Research, Department of Clinical Laboratory, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital, Nanjing, Jiangsu Institute of Cancer Research, Department of Clinical Laboratory, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital, Nanjing, Jiangsu Institute of Cancer Research, Department of Clinical Laboratory, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital, Nanjing, Jiangsu Institute of Cancer Research, Department of Clinical Laboratory, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital, Nanjing, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Department of Surgery, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Department of Surgery, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu Institute of Cancer Research, Department of Clinical Laboratory, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital, Nanjing, Jiangsu Institute of Cancer Research, Department of Clinical Laboratory, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital, Nanjing, Jiangsu Institute of Cancer Research, Department of Clinical Laboratory, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital, Nanjing, Jiangsu Institute of Cancer Research, Department of Clinical Laboratory, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital, Nanjing



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