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Identification of Novel Molecular Network Expression in Acute Myocardial Infarction

[ Vol. 20 , Issue. 5 ]

Author(s):

Marwa Matboli*, Ayman E. Shafei , Sara H.A. Agwa, Sherif Sammir Elzahy, Ahmed K. Anwar, Amr R. Mansour , Ahmed I. Gaber , Ali E.A. Said, Paula Lwis and Marwa Hamdy   Pages 340 - 348 ( 9 )

Abstract:


Background: In the current study, we aimed to analyze the hypothesis that human myocardial- specific extracellular RNAs expression could be used for acute myocardial injury(AMI) diagnosis.

Methodology: We used bioinformatics’ analysis to identify RNAs linked to ubiquitin system and specific to AMI, named, (lncRNA-RP11-175K6.1), (LOC101927740), microRNA-106b-5p (miR-106b- 5p) and Anaphase, promoting complex 11 (ANapc11mRNA). We measured the serum expression of the chosen RNAs in 69 individuals with acute coronary syndromes, 31 individuals with angina pectoris without MI and non-cardiac chest pain and 31 healthy control individuals by real-time reversetranscription PCR.

Results: Our study revealed a significant decrease in both lncRNA-RP11-175K6.1 and ANapc11mRNA expression of in the sera samples of AMI patients compared to that of the two control groups alongside with significant upregulation of miR-106b-5p.

Conclusion: Of note, the investigated serum RNAs decrease the false discovery rate of AMI to 3.2%.

Keywords:

Myocardial infarction, miRNA, lncRNA, serum, diagnosis, extracellular RNAs.

Affiliation:

Medicinal Biochemistry and Molecular Biology Department, Ain Shams University, Faculty of Medicine, Cairo, Biomedical Research Department, Military Medical Academy, Cairo, Clinical Pathology, Medical Ain Shams Research Institute (MASRI), Cairo, Cardiovascular Medicine Department, Ain Shams University, Faculty of Medicine, Cairo, Armed Forces College of Medicine, Cairo, Armed Forces College of Medicine, Cairo, Armed Forces College of Medicine, Cairo, Armed Forces College of Medicine, Cairo, Armed Forces College of Medicine, Cairo, Medicinal Biochemistry and Molecular Biology Department, Ain Shams University, Faculty of Medicine, Cairo



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