Submit Manuscript  

Article Details


Exosomal miR-1298 and lncRNA-RP11-583F2.2 Expression in Hepatocellular Carcinoma

[ Vol. 21 , Issue. 1 ]

Author(s):

Marwa Matboli*, Mariam E. Labib, Hanaa El-Tayeb Nasser, Ahmed H.F. El-Tawdi, Eman K. Habib and Randa Ali-Labib   Pages 46 - 55 ( 10 )

Abstract:


Aim: The aim of this study was to explore the expression of exosomal non-coding RNAs (ncRNAs) in the sera of patients with HCC versus control.

Methods: Firstly, Bioinformatics analysis was conducted to retrieve ncRNAs specific to HCC (hsamiRNA- 1298 and lncRNA-RP11-583F2.2). Afterwards, extraction and characterization of exosomes were performed. We measured the expression of the chosen exosomal RNAs by reverse transcriptase quantitative real-time PCR in sera of 60 patients with HCC, 42 patients with chronic hepatitis C (CHC) infection and 18 healthy normal volunteers.

Results: The exosomal ncRNAs [hsa-miRNA-1298, lncRNA-RP11-583F2.2] had better sensitivity and specificity than alpha-fetoprotein (AFP) in HCC diagnosis.

Conclusion: The exosomal hsa-miRNA-1298, lncRNA-RP11-583F2.2 can be potential biomarkers for HCC diagnosis.

Keywords:

Hepatocellular carcinoma, exosome, miRNA, lncRNA, diagnosis, chronic hepatitis C.

Affiliation:

Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, 11381, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, 11381, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, 11381, General and Plastic Surgery Department, Military Medical Academy, Cairo, Anatomy Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, 11381

Graphical Abstract:



Read Full-Text article