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Genome Sequence of a Highly Virulent pvl-positive Vancomycin intermediate- resistant Staphylococcus aureus Sequence Type 30

[ Vol. 21 , Issue. 2 ]

Author(s):

Raiane C. Chamon, Lucas M. Marques, Jorge Timenetsky, Caio T.C. da Costa Rachid, Rosana B.R. Ferreira, Tamara L.R. de Oliveira, Thais Glatthardt, Lilian de Oliveira Moreira* and Kátia R.N. dos Santos *   Pages 128 - 137 ( 10 )

Abstract:


Background: Staphylococcus aureus isolates expressing the Panton-Valentine Leukocidin (PVL) have been related to a wide range of diseases. Recently, pvl-positive community-associated methicillin-resistant S. aureus belonging to USA1100 (ST30/CC30/SCCmec IV) lineage has emerged in Brazilian hospitals.

Objective: The aim of this work was to sequence the genome of a pvl-positive USA1100 Vancomycin- Intermediate-Resistant S. aureus (VISA) isolate from Rio de Janeiro, Brazil.

Methods: The 13420 genome was sequenced using the HiSeq 2500 platform. The draft genome, plasmids annotation, and genome analysis were performed using RAST. Comparison of the relative pvl gene expression of six S. aureus isolates was performed by qRT-PCR.

Results: The isolate presented the ϕPVL phage codifying for the H2b PVL protein isoform, and another prophage carrying a PVL variant named lukF and lukS-PV.2. The 13420 genome presented a high number of virulence determinants, such as genes codifying for serine-protease proteins, enterotoxins (egc), the immune evasion cluster (IEC), adhesion proteins, spermine/spermidine acetyltransferase gene (blt), superantigen-like proteins, as well as the ica operon. Point mutations at vraS, tcaA, and tcaB genes were detected. Moreover, the PVL mRNA relative expression of the 13420 isolate was five times higher than mRNA PVL levels of the USA300/ST8 reference strain.

Conclusion: We described for the first time the genome sequence of a VISA isolate harboring two pvl-associated genes and other virulence factors that may improve the USA1100/ST30 lineage fitness and impact its pathogenicity and spreading at Brazilian hospitals.

Keywords:

S. aureus, pvl-positive, MRSA, VISA, USA1100/ST30, virulence.

Affiliation:

Laboratorio de Infeccao Hospitalar, Departamento de Microbiologia Medica, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Bahia, Instituto de Ciencias Biomedicas, Universidade de Sao Paulo, Sao Paulo, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Laboratorio de Infeccao Hospitalar, Departamento de Microbiologia Medica, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Laboratorio de Infeccao Hospitalar, Departamento de Microbiologia Medica, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Laboratorio de Infeccao Hospitalar, Departamento de Microbiologia Medica, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Laboratorio de Bacteriologia e Imunologia Clinica, Departamento de Analises Clínicas e Toxicologicas, Faculdade de Farmacia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Laboratorio de Infeccao Hospitalar, Departamento de Microbiologia Medica, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro

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