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The Comparison of Two Single-cell Sequencing Platforms: BD Rhapsody and 10x Genomics Chromium

Author(s):

Caixia Gao*, Mingnan Zhang and Lei Chen   Pages 1 - 8 ( 8 )

Abstract:


The cell is the unit of life for all organisms, and all cells are certainly not the same. So the technology to generate transcription expression or genomic DNA profiles from single cells is crucial. Since its establishment in 2009, single-cell RNA sequencing (scRNA-seq) has emerged as a major driver behind progress in biomedical research. During the course of last three years, several new single-cell sequencing platforms were released. Yet there are only a few systematic comparisons of the advantages and limitations of these commonly used platforms. Here we compare two single-cell sequencing platforms: BD Rhapsody and 10x Genomics Chrominum, including their different mechanisms and some scRNA-seq results obtained with them.

Keywords:

Single-cell sequencing, BD rhapsody, 10x genomics chrominum, transcriptome sequencing, technology comparison

Affiliation:

Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Institute of Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Institute of Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai, Center for Microbiota and Immunological Diseases, Shanghai General Hospital, Shanghai Institute of Immunology, School of Medicine, Shanghai Jiao Tong University, Shanghai



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