Jiawei Li, Yi Zhang, Cheng Yang* and Ruiming Rong Pages 1 - 4 ( 4 )
With the development of single-cell mRNA sequencing (scRNA-seq), researchers have attempted to identify new methods for performing in-depth studies of immune cells. However, the discrepancies between the mRNA levels and the levels of surface proteins have confused many researchers. Here, we report a significant and interesting phenomenon in which the mRNA and protein expression levels were mismatched in immune cells. We concluded that scRNA-seq should be combined with other sequencing methods in single-cell studies (e.g., CITE-seq). The simultaneous assessment of both mRNA and protein expression will enhance the precision and credibility of the results.
Single cell mRNA sequencing, immune cell, mRNA, protein
Department of Urology, Zhongshan Hospital, Fudan University, Shanghai 200032, Biomedical Research Center, Institute for Clinical Sciences, Zhongshan Hospital, Fudan University, Shanghai 200032, Department of Urology, Zhongshan Hospital, Fudan University, Shanghai 200032, Department of Urology, Zhongshan Hospital, Fudan University, Shanghai 200032