Jiawei Li, Yi Zhang, Cheng Yang* and Ruiming Rong* Pages 560 - 563 ( 4 )
With the development of single-cell mRNA sequencing (scRNA-seq), researchers have attempted to identify new methods for performing in-depth studies of immune cells. However, the discrepancies between the mRNA levels and the levels of surface proteins have confused many researchers. Here, we report a significant and interesting phenomenon in which the mRNA and protein expression levels were mismatched in immune cells. We concluded that scRNA-seq should be combined with other sequencing methods in single-cell studies (e.g., CITE-seq). The simultaneous assessment of both mRNA and protein expression will enhance the precision and credibility of the results.
Single-cell mRNA sequencing, immune cell, mRNA, protein, myeloid-derived cells, protein expression.
Shanghai Key Laboratory of Organ Transplantation, Shanghai, Shanghai Key Laboratory of Organ Transplantation, Shanghai, Shanghai Key Laboratory of Organ Transplantation, Shanghai, Shanghai Key Laboratory of Organ Transplantation, Shanghai