Discrepant mRNA and Protein Expression in Immune Cells

Author(s):

Jiawei Li, Yi Zhang, Cheng Yang* and Ruiming Rong*   Pages 560 - 563 ( 4 )

Abstract:

With the development of single-cell mRNA sequencing (scRNA-seq), researchers have attempted to identify new methods for performing in-depth studies of immune cells. However, the discrepancies between the mRNA levels and the levels of surface proteins have confused many researchers. Here, we report a significant and interesting phenomenon in which the mRNA and protein expression levels were mismatched in immune cells. We concluded that scRNA-seq should be combined with other sequencing methods in single-cell studies (e.g., CITE-seq). The simultaneous assessment of both mRNA and protein expression will enhance the precision and credibility of the results.

Keywords:

Single-cell mRNA sequencing, immune cell, mRNA, protein, myeloid-derived cells, protein expression.

Affiliation:

Shanghai Key Laboratory of Organ Transplantation, Shanghai, Shanghai Key Laboratory of Organ Transplantation, Shanghai, Shanghai Key Laboratory of Organ Transplantation, Shanghai, Shanghai Key Laboratory of Organ Transplantation, Shanghai

Graphical Abstract:

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