Submit Manuscript  

Article Details


Understanding the Plant-microbe Interactions in CRISPR/Cas9 Era: Indeed a Sprinting Start in Marathon

Author(s):

Prabhukarthikeyan S. R, Parameswaran C*, Keerthana U, Basavaraj Teli, Prasanth Tej Kumar Jagannadham, Cayalvizhi B, Panneerselvam P, Ansuman Senapati, Nagendran K, Shweta Kumari, Manoj Kumar Yadav, Aravindan S and Sanghamitra S   Pages 1 - 15 ( 15 )

Abstract:


Plant-microbe interactions can be either beneficial or harmful depending on the nature of interaction. Multifaceted benefits of plant-associated microbes in crops are well documented. Specifically, management of plant diseases using beneficial microbes is considered to be eco-friendly and the best alternative for sustainable agriculture. Diseases caused by various phytopathogens are responsible for significant reduction in crop yield and causes substantial economic losses globally. In an ecosystem, there is always an equally daunting challenge for the establishment of disease and development of resistance by pathogens and plants, respectively. In particular, comprehending the complete view of complex biological system of plant-pathogen interactions, co-evolution and plant growth promotions (PGP) at both genetic and molecular levels require novel approaches to decipher the function of genes involved in their interaction. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 (CRISPR-associated protein 9) is a fast, emerging, precise, eco-friendly and efficient tool to address the challenges in agriculture and decipher plant-microbe interaction in crops. Nowadays, CRISPR/Cas9 approach is receiving major attention in the field of functional genomics and crop improvement. Consequently, the present review updates the prevailing knowledge in the deployment of CRISPR/Cas9 techniques to understand plantmicrobe interactions,; genes edited for the development of fungal, bacterial and viral disease resistance,; to elucidate the nodulation processes, plant growth promotion, and future implications in agriculture. Further, CRISPR/Cas9 would be a new tool for the management of plant diseases and increasing productivity for climate resilience farming.

Keywords:

Beneficial microbes, phytopathogens, genome-editing, CRISPR/Cas9, durable disease resistance, plant-microbe interaction

Affiliation:

National Rice Research Institute (ICAR-NRRI), Cuttack, Odisha, National Rice Research Institute (ICAR-NRRI), Cuttack, Odisha, National Rice Research Institute (ICAR-NRRI), Cuttack, Odisha, Banaras Hindu University (BHU), Varanasi, Uttar Pradesh, Central Citrus Research Institute (ICAR-CCRI), Nagpur, Maharastra, National Rice Research Institute (ICAR-NRRI), Cuttack, Odisha, National Rice Research Institute (ICAR-NRRI), Cuttack, Odisha, National Rice Research Institute (ICAR-NRRI), Cuttack, Odisha, Indian Institute of Vegetable Research (ICAR-IIVR), Varanasi, Uttar Pradesh, Indian Institute of Vegetable Research (ICAR-IIVR), Varanasi, Uttar Pradesh, National Rice Research Institute (ICAR-NRRI), Cuttack, Odisha, National Rice Research Institute (ICAR-NRRI), Cuttack, Odisha, National Rice Research Institute (ICAR-NRRI), Cuttack, Odisha



Read Full-Text article