Submit Manuscript  

Article Details


The Caenorhabditis elegans Interactome Mapping Project

[ Vol. 3 , Issue. 2 ]

Author(s):

Jean-Francois Rual, Philippe Lamesch, Jean Vandenhaute and Marc Vidal   Pages 83 - 93 ( 11 )

Abstract:


With the near availability of a cloned Caenorhabditis elegans ORFeome, the development of a high-throughput worm interactome mapping project has now become feasible. After reviewing the increasing interest for C. elegans as a model organism, from ”classical“ biology to the post-genomic era, this article presents the early steps accomplished for the generation of a C. elegans protein interaction map using the twohybrid system. Different approaches have been examined in order to determine the appropriate strategy to screen protein-protein interactions on a large scale. Our version of the two-hybrid system has been used to generate prototype maps for 4 different C. elegans biological modules (vulval development, proteasome, DNA repair / cell cycle and dauer). Those pilot projects helped us developing experimental procedures to avoid most false positive protein interactions while covering a satisfactory proportion of geniune interactions. In silico analysis and experimental validation of the potential protein interactions have already demonstrated biological relevance for some of the networks of interactions obtained.

Keywords:

Caenorhabditis elegans orfeome

Affiliation:

Dana-Farber CancerInstitute, 44 Binney street Boston MA 02115 USA



Read Full-Text article