Jean-Francois Rual, Philippe Lamesch, Jean Vandenhaute and Marc Vidal Pages 83 - 93 ( 11 )
With the near availability of a cloned Caenorhabditis elegans ORFeome, the development of a high-throughput worm interactome mapping project has now become feasible. After reviewing the increasing interest for C. elegans as a model organism, from ”classical“ biology to the post-genomic era, this article presents the early steps accomplished for the generation of a C. elegans protein interaction map using the twohybrid system. Different approaches have been examined in order to determine the appropriate strategy to screen protein-protein interactions on a large scale. Our version of the two-hybrid system has been used to generate prototype maps for 4 different C. elegans biological modules (vulval development, proteasome, DNA repair / cell cycle and dauer). Those pilot projects helped us developing experimental procedures to avoid most false positive protein interactions while covering a satisfactory proportion of geniune interactions. In silico analysis and experimental validation of the potential protein interactions have already demonstrated biological relevance for some of the networks of interactions obtained.
Caenorhabditis elegans orfeome
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